新型防霉剂对墙体霉菌抑制效果的评价
2016-07-10卢鸿侯渊博陈志明
卢鸿 侯渊博 陈志明
摘 要:为检测与评价自配新型防霉剂对墙体霉菌的抑菌、杀菌效果,采集墙体上的霉菌,分离、纯化及18S-rDNA测序鉴定,确定优势菌株;将鉴定出的优势霉菌接种在沙堡培养基中,37 ℃恒温培养2~3 d,采用某市售品牌防霉剂A,不同浓度H2O2及自配防霉剂BIT进行抑菌处理,通过比较抑菌圈大小评估抑制效果。次共分离出3种霉菌属,分别是毛霉菌属、曲霉菌属、毛壳霉菌属;三种方式处理后都能抑制霉菌生长,从抑菌圈大小进行比较得出自配防霉剂的抑菌效果更好。自配新型防霉剂可以用于处理墙体霉变。
关 键 词:防霉剂;霉菌;抑菌效果
中图分类号:TQ 028 文献标识码: A 文章编号: 1671-0460(2016)08-1685-03
Abstract: In order to detect and evaluate the antibacterial effect of new self-made mildew-proof agent, the mildews from the wall were collected, isolated and purified. The dominant mildews were determined by sequencing of 18S rDNA. And then, the dominant mildews were cultured in the PDA agar medium, and incubated at 37 ℃ for 2~3 d. Using the inhibition zone method, the antibacterial effects of market mildew-proof agent (A), self-made mildew-proof agent (BIT), and H2O2 on the mildews were compared. The results show that Mucor, Aspergillus and Chaetomium are the major mildews. The above three kinds of mildew-proof agents show antibacterial effect on the walls mildews, the self-made mildew-proof agent has the best antibacterial effect. So this self-made mildew-proof agent can be used as an anti- mildew regent to deal with the walls mildews.
Key words: mildew-proof agent; mildew; antibacterial effect
溫州属亚热带季风气候,温度适中,雨水充沛。潮湿的环境容易引起墙体发霉。墙体由于霉菌的生长导致装饰涂层褪色乃至脱落,不仅影响了建筑的美观,还破坏了墙体结构,降低墙体的使用寿命[1]。墙体发霉也极大地危害了人类健康,长期接触和吸入霉菌会引发呼吸道炎症及过敏性疾病,产生鼻炎、过敏反应、肺部疾病等症状[2]。目前市售防霉剂种类繁多,但墙体专用防霉剂研究较少。本次实验旨在研发一种新型的墙体专用防霉剂,使其能有效地抑制墙体表面霉菌生长。
1 试验部分
1.1 试验菌种
从我校教学楼霉变的墙体分离得到的毛霉、曲霉和毛壳霉作为试验测试菌株。
1.2 培养基
从我校教学楼霉变的墙体分离得到的毛霉、曲霉和毛壳霉作为试验测试菌株。
1.3 试剂
实验室自配防霉剂BIT;……
