刘芳 杨岩涛 贺福元 刘林 吴双 王宇红 蔡光先

摘要：目的 比较补阳还五汤类方（补阳还五汤和脑健片）中阿魏酸、洋川芎内酯A、藁本内酯在大鼠体内的药代动力学特征。方法 将补阳还五汤类方灌胃颈静脉插管大鼠，采用HPLC-DAD法同时检测3种成分在不同时间点的血药浓度，经DAS2.0计算得单成分的药代动力学参数，再采用总量统计矩（相似度）法整合成整体药代动力学参数并进行比较。结果 3种成分在体内的总量统计参数相差甚远，而3种成分总和的统计矩参数却比较接近，补阳还五汤和脑健片在大鼠体内AUC分别为240.6和133.0，MRT分别为3.192 min和3.259 min，VRT分别为21.59 min2和19.75 min2；补阳还五汤和脑健片中3种成分总量的相似度达到0.977 8。结论 补阳还五汤和脑健片在体内代谢过程具有相似性，中药复方发挥功效依赖于多成分的整体贡献。

关键词：药代动力学；补阳还五汤；脑健片；阿魏酸；洋川芎内酯A；藁本内酯

DOI：10.3969/j.issn.1005-5304.2016.07.021

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0082-05

Comparative Study on Pharmacokinetics of Components of Buyang Huanwu Associated Prescriptions LIU Fang1， YANG Yan-tao1， HE Fu-yuan1， LIU Lin2， WU Shuang1， WANG Yu-hong2， 3， CAI Guang-xian3 （1. Pharmacy School， Hunan University of Chinese Medicine， Changsha 410208， China； 2. The First Hospital of Hunan University of Chinese Medicine， Changsha， 410007 China； 3. National Key Laboratory Co-built by Departments of Herbal Powder and New Drugs of Hunan Province， Traditional Chinese Medicine Powder Technology and Equipment of National and Local Joint Engineering Laboratory， Changsha 410208， China）

Abstract： Objective To study the pharmacokinetic features of ferulaic acid， senkyunolide A and ligustilide in Buyang Huanwu associated prescriptions （Buyang Huanwu Decoction and Naojian Tablets）. Methods HPLC-DAD was applied for simultaneous determination plasma concentration of three ingredients with jugular venous cannula rats after intragastric administration of Buyang Huanwu associated prescriptions. The pharmacolinetic parameters of each ingredient was calculated by DAS2.0， and then the total quantum statistical moment （TQSM） standard similarity was used to measure the overall pharmacokinetics behaviors. Results There were great differences in the three ingredients after the administration of two prescriptions， while the total quantum statistical parameters were very closely. The TQSM pharmacokinetic parameters of the three components in Buyang Huanwu Decoction and Naojian Tablets showed that AUC， MRT， VRT were 240.6 and 133.0， 3.192 min and 3.259 min， 21.59 min2 and 19.75 min2， respectively. The similarity was up to 0.977 8. Conclusion The metabolic processes in vivo of Buyang Huanwu Decoction and Naojian Tablets have similarities. The efficacy of Chinese herbal compounds mostly depends on the multi-components overall contributions.

Key words： pharmacokinetics； Buyang Huanwu Decoction； Naojian Tablets； ferulaic acid； senkyunolide A； ligustilide

基金项目：国家重点基础研究发展计划（2012CB723503）；湖南省中医药科研计划项目（2014135）；湖南省“十二五”中药学重点学科（2011年）

通讯作者：贺福元，E-mail：pharmsharking@tom.com；蔡光先，E-mail：366620183@qq.com

补阳还五汤类方包括补阳还五汤及其精简方（脑健片）。补阳还五汤出自《医林改错·瘫痿论》，由黄芪、川芎、当归等7味中药组成，系清代医家王清任创立的治疗半身不遂辨证属气虚血瘀证的经典名方。脑健片处方是遵循补阳还五汤之方义，秉承“益气祛瘀生新法”，将补阳还五汤精简为黄芪、川芎和地龙，经过二次开发而成的新型制剂。……