段玉敏 张洪娟 刘滨瑞 孟庆杰 刘微

摘要：目的 观察独角莲软胶囊对Hep-2荷瘤裸鼠肿瘤生长的抑制作用及对抑癌基因p53表达的影响，探讨其作用机制。方法 背部皮下接种人肝癌Hep-2细胞悬液制备肿瘤模型。BALB/c裸鼠随机分为模型组、阳性药组和中药高、中、低剂量组，各给药组给予相应药物灌胃，1次/d，连续21 d。监测瘤体积变化，HE染色观察各组瘤组织病理变化，RT-PCR检测各组Hep-2荷瘤裸鼠肿瘤组织p53基因的表达。结果 与模型组比较，各给药组均能抑制肿瘤生长，抑瘤率分别为55.1%、42.8%、30.1%、79.5%，p53基因表达上调；病理观察结果显示，模型组肿瘤细胞数目增多、体积增大、胞质疏松、核深染，各给药组均有不同程度的核固缩、淡染、毛细血管数目减少和局灶性坏死。结论 独角莲软胶囊具有抑制人肝癌生长作用，其机制可能与上调p53基因的表达导致细胞凋亡有关。

关键词：独角莲软胶囊；抗肿瘤；p53基因表达；裸鼠

DOI：10.3969/j.issn.1005-5304.2016.07.016

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0061-04

Inhibitory Effects of Typhonium Giganteum Soft Capsules on Transplantation Tumor and Expression of p53 Gene in Human Liver Cancer Hep-2 Tumor-bearing Nude Mice DUAN Yu-min1， 2， ZHANG Hong-juan2， LIU Bin-rui2， MENG Qing-jie2， LIU Wei2 （1. Shenyang Normal University， Shenyang 110000， China； 2. Heilongjiang Academy of Traditional Chinese Medicine， Harbin 150030， China）

Abstract： Objective To observe the inhibitory effects of Typhonium Giganteum soft capsules on tumor growth in Hep-2 tumor-bearing nude mice and its effects on the expression of tumor suppressor p53 gene； To discuss its mechanism of action. Methods Human liver cancer Hep-2 cell suspension back subcutaneous vaccination was conducted to prepare tumor models. BALB/c nude mice were randomly divided into model group， cisplatinum group， and TCM high-， medium-， and low-dose groups. Each group was given relevant medicine for gavage， once a day for 21 days. Growth changes of tumor volume were monitored； HE staining was used to observe pathological changes of tumor tissues； RT-PCR was used to detect the expression of p53 gene in tumor tissue of Hep-2 nude mice. Results Compared with the model group， all medication groups could inhibit the tumor growth， and the anti-tumor rates were 55.1%， 42.8%， 30.1%， and 79.5%， respectively； the expression of p53 gene increased； pathological observation results showed that the number and the volume of tumor cells increased， with cytoplasm rarefaction and nucleus anachromasis. All medication groups had karyopyknosis， slight staining， and decreasing blood capillary and focal necrosis in varying degrees. Conclusion Typhonium Giganteum soft capsules can inhibit the growth of human liver cancer， and its mechanism may be associated with the up-regulating expression of p53 gene leading to apoptosis.

Key words： Typhonium Giganteum soft capsules； anti-tumor； expression of p53 gene； nude mice

我国肝癌死亡率位居世界第2，男性发病率明显高于女性[1]。目前肝癌患者中大部分失去手术指征，肝功能异常并伴有机体其他症状，对放化疗的敏感性