秦 鹏，谢 鹏，刘广宇，梁宏基，梁淑娃， 黄葵英　(广州市微生物研究所，广东广州 510663)



响应曲面法优化罗伊氏乳杆菌发酵培养基

秦 鹏，谢 鹏，刘广宇，梁宏基，梁淑娃*， 黄葵英(广州市微生物研究所，广东广州 510663)

摘要[目的]优化罗伊氏乳杆菌发酵培养基，提高该菌发酵活菌数。[方法]采用Plackett-Burman以及响应曲面法对罗伊氏乳杆菌发酵培养基进行研究，确定罗伊氏乳杆菌发酵培养基中显著影响发酵活菌数的因素，并通过响应曲面法进行优化，以确定最优发酵培养基组分。[结果]试验表明，所得二次回归模型达到极显著水平，无失拟因素存在。优化后的罗伊氏乳杆菌发酵培养基组成为：葡萄糖 3.09%、酵母粉4.19%、果蔬汁10.03%，在此条件下发酵液活菌数可以达到8.25×109 CFU/mL。[结论]研究证实了响应曲面法可用于优化提高罗伊氏乳杆菌发酵活菌数，为其工业化生产奠定了基础。

关键词罗伊氏乳酸菌；Plackett-Burman设计；响应曲面法

Optimization of Fermentation Medium ofLactobacillusreuteriby Response Surface Methodology

QIN Peng, XIE Peng, LIU Guang-yu, LIANG Shu-wa*et al

(Guangzhou Institute of Microbiology, Guangzhou, Guangdong 510663)

Abstract[Objective] To increase the viable count of submerged fermentation ofLactobacillusreuteri. [Method] Plackett-Burman design and response surface methodology were used to investigate the fermentation medium ofL.reuteri. Factors significantly affected the viable count ofL.reuteriwere detected in fermentation medium. Optimization was carried out by response surface method. The combination of optimal fermentation medium was detected. [Result] The second-order regression models reached extremely significant level without the lack of fit. The optimized culture medium was 3.09% glucose, 4.19% yeast extract powder and 10.03% fruit and vegetable juice. Under this condition, the viable count of fermentation broth was 8.25×109CFU/mL. [Conclusion] Response surface method is suitable for the enhancement of viable count ofL.reuterifermentation broth, and lays foundation for the industrialized production.

Key wordsLactobacillusreuteri; Plackett-Burman design；Response surface method

罗伊氏乳杆菌(Lactobacillusreuteri)是普遍存在于人类和大部分动物肠道中的益生菌，它可以代谢产生有机酸、双乙酰、过氧化氢、细菌素及类细菌素等物质[1-2]，不仅能够调节胃肠道健康，还具有增强免疫力、抗肿瘤、抗癌变及抗衰老等多种功能[3-4]。然而目前，较低的发酵水平制约了罗伊氏乳杆菌的进一步扩大应用。

响应面分析法(response surface methodology，RSM)是一种优化工艺条件的有效方法，中心组合设计(CCD)可用于确定试验因素及其交互作用对指标响应值的影响，精确表述因素和响应值之间的关系[5]。响应面分析法具有试验次数少、周期短，求得的回归方程精确度高等优点，可以研究几个因素的交互作用，是降低生产成本、优化培养条件的一种有效方法，近年来逐渐受到重视，并成功运用到农业、生物、食品等领域[6]。……