陈亮　杨晓凌

(上海市光华中西医结合医院痛风科，上海　200052)



·论著·

骨关节炎患者血清中炎性因子IL-1β、IL-6和COX-2的表达

陈亮杨晓凌

(上海市光华中西医结合医院痛风科，上海200052)

摘要目的： 探讨骨关节炎患者血清中炎性因子白细胞介素-1β(IL-1β)、IL-6、环氧合酶-2(COX-2)的表达。方法： 选择在上海市光华中西医结合医院痛风科就诊的骨关节炎患者72例，分为发病组36例、治愈组36例，以同期门诊就诊的无骨关节炎及其他系统严重疾病的健康人20例作为对照组。各组分别采血、分离血清，用酶联免疫吸附法测定患者血清中炎性因子IL-1β、IL-6、COX-2的表达情况。结果： 骨关节炎患者中，炎性因子IL-1β、IL-6、COX-2呈现高表达趋势；发病组IL-1β、IL-6、COX-2的表达量高于对照组和治愈组(P<0.01)。结论： 炎性因子在骨关节炎患者中高表达，治愈后表达量下降；IL-1β、IL-6、COX-2可作为骨关节的临床诊断和治疗指标。

关键词骨关节炎；炎性因子；白细胞介素-1β；白细胞介素-6；环氧合酶-2

Serum Expression of Inflammatory Cytokines IL-1β, IL-6 and COX-2 in Osteoarthritis Patients

CHENLiangYANGXiaoling

DepartmentofGout,ShanghaiGuanghuaHospital,Shanghai200052,China

AbstractObjective： To explore the serum expression of inflammatory cytokines interleukin-1β(IL-1β), interleukin-6 (IL-6) and cyclooxygenase-2(COX-2) in patients with osteoarthritis.Methods： Seventy-two patients with osteoarthritis, who were admitted to Gout Department of Shanghai Guanghua Hospital, were selected and divided into disease group and cure group. In the same period, twenty healthy individuals, who had no osteoarthritis and severe disease in other systems, were set as control group. Blood sampling and serum separation were conducted in each group. And the serum expression of inflammatory cytokines IL-1β, IL-6, and COX-2 in osteoarthritis patients was determined by enzyme linked immunosorbent assay. Results： In osteoarthritis patients, inflammatory cytokines IL-1β, IL-6 and COX-2 presented a tendency of high expression. The expression levels of IL-1β,IL-6 and COX-2 in diseased group were higher than those in control group and cure group(P<0.01).Conclusions： The inflammatory cytokines present high expression in patients with osteoarthritis and the expression decrease after cure. IL-1β, IL-6 and COX-2 can be used as clinical diagnostic and therapeutic index of osteoarthritis.

Key WordsOsteoarthritis；Inflammatory cytokines；Interleukin-1β;Interleukin-6;Cyclooxygenase-2

2015年调查显示，我国约1.2亿人正在经受骨关节炎的折磨，其中一半以上的≥60岁的老年患有该病，且女性发病率高于男性[1]。骨关节炎是一种无菌性炎性疾病，其发病被认为是一种特异性免疫反应[2]。炎性因子白细胞介素-1β(IL-1β)、IL-6和环氧合酶(COX-2)与免疫反应和疼痛的相关性较高，其中白细胞介素可作为免疫反应的促发剂，而COX-2被认为是疼痛与炎性反应的关键因子。本研究探讨了骨关节炎和IL-1β、IL-6、COX-2的关系，为骨关节炎的特异性诊断和治疗提供理论依据。

1资料与方法

1.1一般资料 选择2013年8月—2015年6月上海市光华中西医结合医院痛风科室确诊且未经任何临床干预和治疗的骨关节炎患者36例(发病组)，已治愈的患者36例(治愈组)，无骨关节炎及其他系统严重疾病的健康人20例(对照组)。……