胃癌中凋亡抑制蛋白的表达与化疗耐药的关系

2016-01-25曹晓静白玉洁宋晓翠杜丽艳杨德志马超宋晓昕张春山

河北医药 2015年16期

关键词：胃癌

曹晓静　白玉洁　宋晓翠　杜丽艳　杨德志　马超　宋晓昕　张春山

胃癌中凋亡抑制蛋白的表达与化疗耐药的关系

曹晓静白玉洁宋晓翠杜丽艳杨德志马超宋晓昕张春山

【摘要】目的探讨胃癌组织中Livin、Survivin的表达与化疗药敏性的关系。方法收集102例胃癌标本，用免疫组织化学检测Livin和Survivin的表达。新鲜胃癌组织制备单细胞悬液，分别加入6种化疗药物(奥沙利铂、氟尿嘧啶、顺铂、紫杉醇、阿霉素、环磷酰胺)培养48 h，MTT法观察药物作用后癌细胞活性变化。结果Livin、Survivin在胃癌组织中表达明显高于组织(P＜0.05)。Livin在胃癌组织中高表达时，与奥沙利铂、氟尿嘧啶、顺铂耐药性有关(P＜0.05)。Survivin高表达时，对顺铂、阿霉素、环磷酰胺耐药性有关(P＜0.05)。结论肿瘤中Livin、Survivin的过表达与部分化疗药物耐药有关。

【关键词】胃癌;化疗药物; Livin; Survivin

【中图分类号】R 735.2

【文献标识码】A

【文章编号】1002－7386(2015)16－2415－03

doi:10.3969/j.issn.1002－7386.2015.16.004

【Abstract】Objective To investigate the correlation between the expression of Livin，Survivin and chemotherapeutic drug resistance in gastric cancer.Methods One hundred and two specimens of gastric cancer were colleted，and the expression levels of Livin，Survivin were detected by immunohistochemistry.The single cell suspension was prepared with fresh tumor tissue，then 6 kinds of chemotherapeutic drugs including oxaliplatin，fluorouracil，cisplatin，paclitaxel，adriamycin，cyclophosphamide were added into the suspension which was cultured for 48 hours，finally the changes of cancer cell activity were observed bymTT.Results The expression levels of Livin and Survivin in gastric cancer tissue were significantly higher than those of normal tissues(P＜0.05).The high expression of Livin in gastric cancer was correlated to the drug resistance of oxaliplatin，fluorouracil，cisplatin(P＜0.05).The high expression of Survivin was related with the drug resistance of cisplatin，adriamycin，cyclophosphamide(P＜0.05).Conclusion The overexpression of Livin，the and Survivin is correlated to the part of chemotherapeutic drug resistance in gastric cancer.

收稿日期:(2015－01－20)

作者单位: 054001河北省邢台市第一医院胃肠外科(曹晓静、宋晓翠、杜丽艳、杨德志、马超、张春山)，病理科(宋晓昕);河北省邢台市第二医院外科(白玉洁)

Study on the expression of Livin，Survivin and chemotherapeutic drug resistance in gastric cancer

CAO Xiaojing*，BAI Yujie，SONG Xiaocui*，et al.*Department of Gastrointestinal Surgery，The First Hospital of Xingtai City，Hebei，Xingtai 054001，China

【Key words】gastric carcinoma; chemotherapeutic drug; Livin; Survivin

在我国早期胃癌诊断率低，胃癌经常到晚期才得到诊断，因而化疗在胃癌治疗中占据非常重要地位。但化疗耐药性成为阻碍化疗效果提高的主要因素之一。胃癌耐药机制的研究一直是国内外的热点，细胞凋亡异常是耐药机制之一。我们选取102例胃癌组织和11例正常胃组织，检测了凋亡抑制蛋白Livin、Survivin的表达情况，并分析与6种常见化疗药物耐药性的关系。