魏世平　张俊会

姜黄素对裸鼠人胃癌细胞MMP-2和CD44v6在mRNA水平表达的影响

魏世平张俊会

【摘要】目的观察姜黄素对胃癌组织中MMP-2和CD44v6在mRNA水平表达的影响，探讨其抑制胃癌细胞转移的作用机制。方法以SGC-7901胃癌细胞株建立胃癌裸鼠原位移植瘤模型，将裸鼠随机分为对照组及姜黄素组，每组12只。观察裸小鼠胃癌种植后肿瘤生长及转移灶情况，用原位杂交方法检测肿瘤组织中MMP-2mRNA和CD44v6mRNA表达的变化。结果所有荷瘤鼠胃壁均有肿瘤生长，荷瘤率100%，姜黄素组肝、腹腔和淋巴结的转移比对照组明显减少(P＜0.05);姜黄素治疗组瘤体中MMP-2mRNA和CD44v6mRNA的表达明显下调(P＜0.05)。结论姜黄素具有抑制人胃癌裸鼠原位移植瘤转移的作用，其作用机制可能与下调MMP-2mRNA和CD44v6mRNA的表达有关。

【关键词】姜黄素;胃癌;mmP-2; CD44v6

【中图分类号】R 735.2

【文献标识码】A

【文章编号】1002－7386(2015)16－2412－04

doi:10.3969/j.issn.1002－7386.2015.16.003

【Abstract】Objective To investigate the effects of curcumin on the expression ofmmP-2mRNA and CD44v6mRNA in human gastric cells，and to explore the possible actionmechanisms of curcumin in inhibitingmetastasis of gastric cancer.Methods The orthotopic transplantation tumormodels were established in nudemice with SGC-7901 gastric cancer cell line，then the nudemice were randomly divided into control group and curcumin group，with 12mice in each group.The tumor growth andmetastasis condition were observed，and the expression levels ofmmP-2mRNA and CD44v6mRNA in tumor tissue were detected by in situ hybridization.Results The successful rate of orthotopic transplantation tumor was 100%.Themetastases of liver，peritoneal cavity and lymph node in curcumin group were significantly less than those in control group(P＜0.05)，moreover，the expression levels ofmmP-2mRNA and CD44v6mRNA in curcumin group were significantly down-regulated，as compared with those in control group(P＜0.05).Conclusion Curcumin can inhibit gastric cancermetastasis in nudemice orthotopic transplantation tumor，and its actionmechanismmight be related to down-regulation of the expression levels ofmmP-2mRNA and CD44v6mRNA.

收稿日期:(2015－02－06)

作者单位: 054000河北省邢台市，邢台医学高等专科学校病理教研室

Influence of curcumin on the expression ofmmP-2mRNA and CD44v6mRNA in human gastric cancer cells in nudemicemodel

WEI Shiping，ZHANG Junhui.Xingtaimedical College，Hebei，Xingtai 054000，China

【Key words】curcumin; gastric cancer;mmP-2; CD44v6

胃癌是常见的消化道恶性肿瘤，在我国，每年死于胃癌患者达16万人，占全部恶性肿瘤死亡人数的23%。胃癌的发生、发展是多因素、多阶段、多基因变异所致的癌变过程。胃癌的单纯手术切除，可获治愈的仅限早期胃癌且不伴有任何转移灶者，有转移灶的早期胃癌及全部进展期胃癌，内科治疗占有重要地位。姜黄素是从植物姜黄的根茎中提取的主要有效成分，大量研究表明，姜黄素具有抗氧化、抗炎和抗肿瘤活性［1］。在肿瘤的发生发展过程中，姜黄素通过多种通路和靶点相互作用，发挥抗肿瘤作用［2］。但其抗肿瘤机制较为复杂，至今仍未完全阐明。……