张国英，　钮晓红，　徐卫平，　夏　厦

(南京中医药大学附属南京市中西医结合医院检验科,江苏 南京 210014)

淋巴结核患者外周血CD4+CD25highFoxP3+调节性T淋巴细胞以及血浆IFN-γ和IL-10水平及其临床意义

张国英，钮晓红，徐卫平，夏厦

(南京中医药大学附属南京市中西医结合医院检验科,江苏 南京 210014)

摘要：目的研究淋巴结核患者外周血CD4+CD25highFoxP3+调节性T淋巴细胞(Treg)和干扰素γ(IFN-γ)、白细胞介素10(IL-10)水平的变化及临床意义。方法采用流式细胞仪对100例淋巴结核患者及30名正常人外周血CD4+CD25highFoxP3+Treg和IFN-γ、IL-10水平进行检测。结果淋巴结核患者组与正常对照组比较，外周血CD4+CD25highFoxP3+Treg和IL-10水平均升高(P<0.05)，而IFN-γ水平则降低(P<0.05)。在干酪样型、增殖型和混合型3种类型的淋巴结核病之间各指标差异均无统计学意义(P>0.05)。结论淋巴结核患者细胞免疫功能明显异常。CD4+CD25highFoxP3+Treg和IFN-γ、IL-10在淋巴结核的发病过程中发挥了重要作用。

关键词：CD4+CD25highFoxP3+调节性T淋巴细胞；干扰素γ；白细胞介素10；淋巴结核

中图分类号：

文章编号：1673-8640(2015)01-0031-05R446.63

文献标志码：码：A

DOI：10.3969/j.issn.1673-8640.2015.01.008

Abstract:ObjectiveTo study the changes of peripheral blood CD4+CD25highFoxP3+regulatory T lymphocytes (Treg) and interferon gamma (IFN-γ), interleukin 10 (IL-10) levels of patients with lymph tuberculosis and their clinical significance. MethodsThe levels of peripheral blood CD4+CD25highFoxP3+Treg, IFN- γ and IL-10 of 100 lymph tuberculosis patients and 30 healthy subjects were determined by flow cytometry. ResultsCompared with the healthy controls, the levels of peripheral blood CD4+CD25highFoxP3+Treg and IL-10 levels in lymph tuberculosis group increased with statistical significance (P<0.05), while IFN-γ level decreased (P<0.05). Among the caseous, proliferation and mixed types of lymph tuberculosis, there was no significant difference (P>0.05). ConclusionsCellular immune function is significantly abnormal in patients with lymph tuberculosis. CD4+CD25highFoxP3+Treg, IFN- γ and IL-10 play important roles in the onset process of lymph tuberculosis.

基金项目：南京市医学科技发展项目(QYK11146)

作者简介：张国英，女，1978年生，硕士，副主任技师，主要从事微生物与免疫学研究。

收稿日期：(2014-01-21)

Levels of CD4+CD25highFoxP3+regulatory T lymphocytes in peripheral blood and plasma IFN-γ, IL-10 of patients with lymph tuberculosis and their clinical significanceZHANGGuoying,NIUXiaohong,XUWeiping,XIAXia.(DepartmentofClinicalLaboratory,NanjingHospitalofIntegratedTraditionalandWesternMedicine,NanjingUniversityofTraditionalChineseMedicine,JiangsuNanjing210014,China)

Key words: CD4+CD25highFoxP3+regulatory T lymphocyte; Interferon gamma; Interleukin 10; Lymph tuberculosis

结核病是由结核分枝杆菌(Mycobacteriumtuberculosis，MTB)感染引起的慢性传染病，其中，淋巴结核病是指发生于全身淋巴结的结核病，如颈部淋巴结核、肠系膜淋巴结核等，约占结核病的10%。对这类结核病的研究，目前仍相对较少。临床上因此漏诊率、误诊率较高[1]。

结核分枝杆菌为胞内寄生菌，机体的免疫清除作用主要依赖于分泌干扰素γ(interferon gamma，IFN-γ)的CD4+和CD8+效应性T细胞。CD4+CD25highFoxP3+调节性T淋巴细胞(regulatory T lymphocyte，Treg)是SAKAGUCHI等首先描述的在胸腺内发育成熟的抑制自身免疫和移植排斥反应的一群细胞，占CD4+T细胞总数的5%~10%[2]，能够分泌白细胞介素10(interleukin 10，IL-10)等抑制因子，从而发挥免疫调节作用[3]。……