涂洋，陈光亮，曹珊，陈晓翔(上海交通大学医学院附属仁济医院，上海200001)

系统性红斑狼疮患者外周血白细胞唾液酸转移酶表达变化及机制探讨

涂洋，陈光亮，曹珊，陈晓翔
(上海交通大学医学院附属仁济医院，上海200001)

摘要:目的观察系统性红斑狼疮( SLE)患者外周血白细胞唾液酸转移酶( ST8SIA)的表达变化，并探讨其机制。方法选择SLE患者(观察组)和健康志愿者(对照组)各24例，采用RT-PCR法检测外周血白细胞ST8SIA1 ～6 mRNA;对有统计学意义的ST8SIA mRNA，采用基因芯片技术分析血浆中靶向其3'非翻译区( 3'UTR)的miRNA。结果两组外周血白细胞均无ST8SIA2、ST8SIA3、ST8SIA5 mRNA表达;观察组ST8SIA1、4、6 mRNA相对表达量分别为1.524±0.229、13.780±1.669、2.238±0.387，对照组分别为1.129±0.106、1.087±0.089、0.550± 0.054;两组ST8SIA4、6 mRNA比较，P均＜0.05。靶向调节ST8SIA4 mRNA的miRNA表达下降12条、升高1条，靶向调节ST8SIA6 mRNA的miRNA表达下降、升高各4条。结论SLE患者外周血白细胞中ST8SIA4、ST8SIA6 mRNA表达升高，与对其进行靶向调控的miRNA表达异常有关。

关键词:系统性红斑狼疮;α2，8-唾液酸转移酶;微小核糖核酸;聚合酶链反应;基因芯片

Expression changes of sialyltransferase in peripheral blood leucocytes of patients with systemic lupus erythematosus and the possible mechanism.

TU Yang，CHEN Guang-liang，CAO Shan，CHEN Xiao-xiang
( Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine，Shanghai 200001，China)

Abstract:Objective To observe the expression changes of sialyltransferase ( ST8SIA) in the peripheral blood leucocytes of patients with systemic lupus erythematosus ( SLE) and to investigate the possible mechanism.Methods ST8SIA1-6 was detected by RT-PCR in the peripheral blood leucocytes in 24 SLE patients and 24 normal donors.We analyzed the miRNAs that targeting 3untranslated region ( UTR) by microarray chip.Results The expression of ST8SIA2，ST8SIA3 and ST8SIA5 was not detected in the peripheral blood leucocytes of the two groups.The relative expression levels of ST8SIA1 mRNA，ST8SIA4 mRNA and ST8SIA6 mRNA in the observation group were 1.524± 0.229，13.78±1.669 and 2.238±0.387，and they were 1.129±0.106，1.087±0.089 and 0.550±0.054 in the control group.The difference in ST8SIA4 mRNA and ST8SIA6 mRNA of the two groups was statistically different ( all P＜0.05).There were 12 down-regulated plasma miRNAs that regulated the 3UTR of ST8SIA4 mRNA and 1 up-regulated plasma miRNA.There were 4 plasma miRNAs that made the 3UTR of ST8SIA6 mRNA down-regulated and 4 plasma miRNAs up-regulated.Conclusion The expression levels of ST8SIA4 mRNA and ST8SIA6 mRNA in patients with SLE are increased，which may be related with the abnormal expression of plasma miRNAs that can regulate the 3UTR of ST8SIA4 mRNA and ST8SIA6 mRNA.

Key words:systemic lupus erythematosus;α2，8-sialyltransferases; miRNA; polymerase chain reaction; microarray

系统性红斑狼疮( SLE)是一种原因未明，以多系统或器官病变和血清中出现大量致病性自身抗体为特征的自身免疫性疾病［1］。研究发现，SLE患者血浆中的IgG-Fc段唾液酸化程度比正常人降低，并且与SLE活动度呈负相关［2，3］。唾液酸转移酶( ST8SIA)是一种唾液酸糖基转移酶，共有6种亚型( ST8SIA1～6)。MicroRNAs( miRNAs)是在真核生物体内广泛存在的一类具有调控功能的小RNA。

成熟的miRNAs通过碱基互补配对方式识别特定mRNA的3'非翻译区( 3'UTR)，使其降解或阻遏其翻译过程，从而调控基因表达［4，5］。研究发现很多疾病与miRNA表达水平有关，如心血管疾病、血液系统疾病、自身免疫性疾病等［6］。……