郭路生　张丽娜　杨宁江

树突状细胞疫苗对小鼠膀胱癌模型抑瘤效应研究

郭路生1张丽娜2杨宁江1

【摘要】目的 探讨负载MB49细胞粗提全抗原的树突状细胞（DC2.4）疫苗对C57BL/6小鼠膀胱癌MB49皮下移植瘤模型的抑瘤效果。方法 制备负载MB49细胞粗提全抗原的DC疫苗；建立小鼠MB49膀胱癌皮下移植瘤模型。造模后第7 d、14 d，疫苗组小鼠于右前腋皮下注射疫苗，PBS对照组相应注射PBS，正常组正常饲养。造模第21 d，测量肿瘤体积和重量。应用免疫组化染色方法检测肿瘤组织内CD4+、CD8+细胞数。应用SPSS13.0统计分析测量数据。结果 DC疫苗组和PBS对照组小鼠肿瘤体积随时间呈现不同程度增长趋势，DC疫苗组缓于PBS对照组，差异有统计学意义（P＜0.01）；造模21 d，DC疫苗组小鼠肿瘤重量小于PBS对照组，差异有统计学意义（P＜0.01）；免疫组化染色检测小鼠肿瘤组织内CD4+、CD8+T细胞数，DC疫苗组CD4+、CD8+T细胞数高于PBS对照组，差异有统计学意义（P＜0.01）。结论 DC2.4树突状细胞疫苗抑制了模型小鼠肿瘤的生长，起到了抗肿瘤的免疫学效应。

【关键词】树突状细胞疫苗；MB49细胞；膀胱癌模型；免疫组织化学染色

作者单位：1 132013吉林，吉林医药学院附属医院；2 132013吉林，吉林医药学院护理学院

基金资助：吉林市科技计划项目（201133103）

Study on Tumor Suppressor Effect of Dendritic Cell Vaccine in Mouse Bladder Cancer Model

GUO Lusheng1ZHANG Li’na2YANG Ningjiang1, 1 The affiliated hospital of Jilin Medical University, Jilin 132013, China, 2 Department of nursing, Jilin Medical University, Jilin 132013, China

[Abstract]Objective To investigate the antitumor effect of dendritic cell vaccine against C57BL/6 cells loaded with MB49 cells in MB49 mice. Methods DC vaccine against MB49 cells were prepared by loading MB49 cells, and the model was established in mice. After making the model, the 7 d, 14 d, the vaccine were injected in right anterior axillary of vaccine group mice, and the mice of PBS control group were injected with PBS, normal feeding to normal group. 21 d after modeling, measurement of the volume and weight of the tumor. The number of CD4+and CD8+

cells in tumor tissues was detected by immunohistochemical staining. SPSS13.0 statistical analysis of measurement data. Results The tumor volume of DC vaccine group and PBS control group showed different degrees of growth trend, DC vaccine group was significantly slower than that in 21 d control group(P<0.01). The tumor weight was significantly less than that in DC group(P<0.01). In tumor tissue of CD4+and CD8+

T cell number and DC vaccine group of CD4+, CD8+

T cell number higher than the PBS control group. The difference was statistically significant(P < 0.01). Conclusion DC2.4 dendritic cell vaccine inhibits tumor growth in mouse model, which has the effect of anti-tumor.[Key words]Dendritic cell vaccine, MB49 cells, Bladder cancer models, Immunohistochemical staining

膀胱癌是泌尿系统发病率最高的恶性肿瘤，且有逐年增高的趋势；其发病率仅次于前列腺癌，居泌尿系统肿瘤的第二位[1]。膀胱癌也被认为是最有希望通过免疫和基因疗法治疗的疾病[2]。近年来，DC疫苗在实验室中广泛应用于治疗肿瘤的研究，在临床上，也已试验性的应用于肿瘤的治疗[3-4]。……