软枣猕猴桃叶片光系统Ⅱ活性对不同温度的响应

王振兴1,艾军1*,陈丽2,范书田1,何伟1,秦红艳1,赵滢1

(1 中国农业科学院特产研究所,长春 130112;2 吉林农业大学 园艺学院,长春 130112)

摘要:以软枣猕猴桃“魁绿”为试验材料,利用快速叶绿素荧光诱导动力学曲线分析技术(JIP-test)研究了热胁迫处理对植株叶片光系统Ⅱ活性的影响。结果显示:(1)软枣猕猴桃叶片最大光化学效率(Fv/Fm)在35～48 ℃的范围内并没有明显变化,只有当温度升高到52 ℃时才显著下降。(2)随着温度升高,叶绿素荧光诱导动力学曲线中J点和I点的相对可变荧光Vj和Vi呈显著下降趋势,在52 ℃又显著升高,而K点的相对可变荧光Vk则逐渐上升;叶片捕获的激子将电子传递到电子传递链中QA-下游电子受体的概率(ψ0)随着温度升高而逐渐上升,但在52 ℃时显著下降。(3)随着热胁迫时间的延长,Vj和Vi随时间延长而升高,ψ0则下降,电子传递链受体侧受到了严重的抑制。研究表明,高温显著抑制了软枣猕猴桃叶片PSⅡ电子传递链供体侧和受体侧的活性,但PSⅡ的供体侧比受体侧对高温更加敏感;JIP-test测定的相关参数能有效地评价不同温度对软枣猕猴桃光系统活性的影响。

关键词:软枣猕猴桃;高温胁迫;光系统;JIP-test

收稿日期:2014-07-07;修改稿收到日期:2014-12-03

基金项目:吉林省科技发展计划(20130102046JC);吉林省科技发展计划(20140204034NY)

作者简介:王振兴(1982-),男,助理研究员,在读博士研究生,主要从事果树生理研究。E-mail:zhenxinghd@aliyun.com

通讯作者:*艾军,博士,研究员,主要从事果树生理生化研究。E-mail:aijun1005@163.com

中图分类号:Q945.79 文献标志码:A

Activity of Photosystems Ⅱ in Leaves ofActinidiaarguta

underdifferent Temperature Treatments

WANG Zhenxing1,AI Jun1*,CHEN Li2,FAN Shutian1,HE Wei1,QIN Hongyan1,ZHAO Ying1

(1 Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Changchun,Jilin 130112,China;2 Jilin Agricutural University,Changchun,Jilin 130112,China)

Abstract:The effects of heat stress on activities of photosystem Ⅱ in leaves of Actinidia arguta (Sieb.et Zucc.) Planch.exmiq.(Kuilü,a cultivar) were studied by chlorophyll a fluorescence transient and JIP-test.The results showed that:(1)Fv/Fm of leavesdid not significantly change at 35-48 ℃,butdecreased at 52 ℃.(2)With increase of leaf temperature,the relative variable fluorescence at J and I steps of fluorescence transient (Vj and Vi) significantlydecreased,but increased at 52 ℃,the relative variable fluorescence Vk increased gradually.(3)With the increase of heat treatment time,the Vj and Vi increased gradually,but Ψodecreased gradually,indicating that the acceptor side of PSⅡ wasdamaged severely.In summary,activity of thedonor side and the acceptor side of PSⅡ was inhibited by high temperature.Thedonor side wasmore sensitive to heat stress.JIP-test parameters can evaluate effectively activity of photosystems Ⅱ in leaves of A.arguta underdifferent temperatures.

Key words:Actinidiaarguta(Sieb.et Zucc.) Planch.exmiq.;heat stress;photosystem;JIP-test

软枣猕猴桃……