脂血对Sysmex XT—1800i检测血液分析结果的影响
2015-08-29王宇
王宇
[摘要]目的 探讨脂血对Sysmex XT-1800i检测血液分析指标的影响。 方法 取无溶血、脂血和黄疸的全血标本40例,每例标本分装成4管,每例第1管组成对照组(n=40),其余3管分别添加脂肪乳剂制成低水平脂血标本组(n=40)、中水平脂血(n=40)和高水平脂血(n=40)。用Sysmex XT-1800i测定各组血液分析指标。结果 低水平脂血组 血标本WBC、RBC、Hb、MCV、MCH、MCHC、PLT测定结果与对照组比较,差异无统计学意义(P>0.05)。中水平脂血组Hb、MCH、MCHC测定结果与对照组比较,差异有统计学意义(P<0.05)。高水平脂血组Hb、MCV、MCH、MCHC测定结果与对照组比较,差异有统计学意义(P<0.05)。结论 中度或重度脂血可影响Sysmex XT-1800i检测血液分析结果。
[关键词]脂血;Sysmex XT-1800i;血液分析;影响
[中图分类号]R4 [文献标识码]A [文章编号]1674-0742(2015)04(b)-0182-02
The Effect of Lipohemia on the Detection Results of Blood Analysis by Sysmex XT-1800i
WANG Yu
Clinical Laboratory, Civil Aviation College Hospital of China, Guanghan, Sichuan Province, 618307 China
[Abstract] Objective To discuss the effect of lipohemia on the detection results of blood analysis by Sysmex XT-1800i. Methods 40 cases of blood samples were selected without hemolysis, lipohemia and jaundice. Each case was packed into 4 tubes. The first tube of each case was used for control group(n=40), and the remaining 3 tubes are respectively added by fat emulsion and prepared for low level of lipohemia samples group(n=40), the middle level of lipohemia(n=40) and high the level of lipohemia (n=40). Blood analysis markers in each group were detected by Sysmex XT-1800i. Results The values of RBC, Hb, MCV, WBC, MCH, MCHC, PLT showed no significant differences in low level of lipohemia group when compared to the control(P>0.05). The values of Hb, MCH, MCHC showed significant differences in middle level of lipohemia group when compared to the control(P<0.05). The values of Hb, MCV,MCH, MCHC showed significant differences in high level of lipohemia group when compared to the control(P<0.05). Conclusion Middle or high level of lipohemia has an effect on the detection results of blood analysis by Sysmex XT-1800i.
[Key words] Lipohemia; Sysmex XT-1800i; Blood analysis; Effect
Sysmex XT-1800i 是一台先进的全自动血液分析仪,采用半导体流式细胞分析系统、结合细胞化学染色技术、荧光检测技术,性能稳定,在临床检验中得到了广泛应用[1]。由于血液成分复杂,溶血和脂血因素对血清生化指标的影响较为明确,但对血液分析指标的影响罕见报道[2-3]。溶血对血液分析的影响报道较多,但脂血对血液分析指标的影响却较少报道[4-5]。该研究选取该院2014年11月—12月健康体检人群的EDTA抗凝全血标本40例作为研究对象,探讨脂血因素对Sysmex XT-1800i检测血液分析指标的影响。现报道如下。
1 资料与方法
1.1 一般资料
选择该院2014年11月—12月健康体检人群的EDTA抗凝全血标本40例,所有标本无溶血、脂血和黄疸。研究对象年龄分布在19~45岁之间,其中男性20例,女性20例。
1.2 仪器、试剂和方法
1.2.1 仪器和试剂 Sysmex XT-1800i及血液分析检测试剂购自Sysmex公司;日立7080全自动生化分析仪购自日立公司;TG检测试剂购自四川迈克生物诊断有限公司。脂肪乳剂购自德国 B.Braun 公司。EDTA-K2真空采血管购自BD公司。……p>
