马永莉++++++陈燕丽++++++郝金娟++++++阮玉华++++++邵一鸣++++++洪坤学

[摘要] 目的 探讨人类免疫缺陷病毒Ⅰ型（HIV-1）B′亚型感染者人类白细胞抗原（HLA）-A、-B、-C位点等位基因的分布及其对病毒载量的影响。 方法 对146例HIV-1B′亚型感染者采用聚合酶链反应-序列特异性引物（PCR-SSP）扩增方法进行HLA-A、-B、-C位点等位基因检测，计算各位点等位基因的频率及其对感染者病毒载量的影响。 结果 在146例HIV-1B′亚型感染者中分别检出HLA-A位点14个等位基因，-B位点24个等位基因，-Cw位点12个等位基因，其中频率大于0.1的等位基因为HLA-A*02，-A*11，-A*24，-A*30，-B*13，-B*40，-B*51，-Cw*03，-Cw*06，-Cw*08；HLA-A、-B、-C等位基因纯合子携带者的病毒载量高于杂合子（P=0.0013）；HLA-A*03（P=0.0314）、-A*30（P=0.0072）、-B*13（P=0.0087）、-Cw*06（P=0.0145）等位基因携带者具有较低病毒载量。 结论 HIV-1B′亚型感染者HLA-A*03、-A*30、-B*13、-Cw*06等位基因与低病毒载量相关。

[关键词] 人类免疫缺陷病毒；人类白细胞抗原；病毒载量；感染

[中图分类号] R117[文献标识码] A[文章编号] 1673-7210（2014）06（c）-0009-04

Impact of HLA class Ⅰ alleles on viral load in HIV-1 B′ infected individuals

MA Yongli CHEN Yanli HAO Jinjuan RUAN Yuhua SHAO Yiming HONG Kunxue▲

National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Beijing 102206, China

[Abstract] Objective To study the distribution of HLA-A, -B, and C alleles in HIV-1 B′infected individuals and to analyze its impact on HIV-1 viral load. Methods 146 HIV-1 infected individuals were recruited and their HLA-A, -B, and C alleles were genotyped using PCR-SSP technique. Allele frequencies and association of HLA-A, -B, and C alleles with HIV-1 viral loads were also analyzed. Results 14 HLA-A allels, 24 HLA-B allels, and 12 HLA-Cw allels were detected in 146 HIV-1 B' infected individuals. Of them, HLA-A*02, -A*11, -A*24, -A*30, -B*13, -B*40, -B*51, - Cw*03, -Cw*06, -Cw*08 were among the most common alleles with frequencies above 0.1; subjects homozygous at HLA class Ⅰ loci had higher viral loads (P=0.0013), subjects carrying HLA-A*03 (P=0.0314), -A*30 (P=0.0072), -B*13 (P=0.0087) and -Cw*06 (P=0.0145) alleles had lower viral loads. Conclusion HLA-A*03, -A*30, -B*13 and -Cw*06 alleles may associate with lower viral loads in HIV-1 B′infected individuals.

[Key words] Human immunodeficiency virus; Human leukocyte antigen; Viral load; Infection

人类白细胞抗原（human leukocyte antigen，HLA）基因影响人类免疫缺陷病毒（human immunodeficiency virus，HIV）感染者的病毒复制，有研究报道携带HLA-B*27，-B*57等位基因的HIV感染者病毒载量较低，多表现为缓慢疾病进程，而HLA-B*35，-B*08，-B*56等位基因则与HIV-1感染者的疾病快速进展相关[1-2]。HLA不同等位基因对HIV病毒复制影响的差异可能与其提呈HIV保护性或非保护性抗原表位，从而激发具有细胞毒作用的CD8+T细胞（cytotoxic T lymphocyte，CTL）免疫应答的能力不同有关[3-4]。然而HLA基因对HIV-1病毒复制和感染者疾病进展的作用受不同人群特定的遗传背景的影响，目前在不同地区不同种族人群中的研究结果并不完全一致[5]。本研究探讨了146例未接受抗病毒治疗的B′亚型HIV-1感染者的HLA Ⅰ类等位基因分布，并分析了该感染人群HLA Ⅰ类等位基因对HIV-1病毒载量的影响。……