蒋苹+许红+王中弥+于璐+孙洁+曹良杰+周亚丽+栗琼

【摘要】目的探讨卵巢子宫内膜异位囊肿、子宫内膜异位症相关的卵巢癌(卵巢子宫内膜样癌和卵巢透明细胞癌)中抑癌基因PTEN蛋白和凋亡抑制基因Survivin蛋白表达及意义。方法利用免疫组织化学二步抗法检测卵巢子宫内膜异位囊肿20例、子宫内膜异位症相关的卵巢癌17例, 正常子宫内膜20例中PTEN蛋白、Survivin蛋白的表达。结果PTEN蛋白在正常子宫内膜的阳性表达低于子宫内膜异位症相关的卵巢癌、卵巢子宫内膜异位囊肿, 差异具有统计学意义(P<0.05)。Survivin蛋白的趋势与PTEN相反, 差异具有统计学意义(P<0.05)。PTEN、Survivin蛋白表达与分期、分化程度, 差异无统计学意义(P>0.05)。PTEN、Survivin蛋白表达无明显相关性(r=0.143, P>0.05)。结论卵巢子宫内膜异位囊肿发生恶变的过程中可能与PTEN、Survivin蛋白表达异常有关, 可能PTEN蛋白表达异常更有意义。

【关键词】PTEN; Survivin；子宫内膜异位症; 卵巢肿瘤; 基因表达

Expression of PTEN and Survivin protein in ovarian cancers associated to endometriosis and its clinical significance JIANG Ping, XU Hong, WANG Zhong-mi, et al. Department of Gynecology, Kunming First People's Hospital, Kunming 650011, China

【Abstract】Objective To investigate the expression of PTEN protein and Survivin protein in endometriosis associated ovarian cancers(EAOCs) and their significance and correlation. MethodsImmunohistochemical method was used to detect the expression of PTEN and Survivin in 17 cases of EAOCs, 20 cases of endometrial cyst of the ovary and 20 cases of normal endometrium. ResultsThe positive rates of PTEN immunostaining showed higher expression in normal endometrium than the others. Respectively, there were significant difference between the three groups(P<0.05). The tendency of Survivin expression was opposite to that of PTEN. The positive expression of PTEN and Survivin was not correlated with clinical stage and differentiation degree of cancer cells (P>0.05). The positive expression of PTEN was not correlated with Survivin. ConclusionPTEN and Survivin protein may be associated between endometrial cyst of the ovary and ovarian cancer associated to endometriosis, and PTEN protein is more significant.

【Key words】PTEN; Survivin; Endometriosis; Ovarian neoplasms; Gene expression作者单位：650011昆明市第一人民医院妇科(蒋苹许红王中弥曹良杰周亚丽栗琼), 病理科(于璐孙洁)子宫内膜异位症(endometriosis, EMs) 最常异位于卵巢, 是育龄期妇女的常见良性病, 但具有类似恶性肿瘤的行为, 并可发生恶变。卵巢子宫内膜异位症恶变的主要细胞学类型为子宫内膜样癌和透明细胞癌, 称为子宫内膜异位症相关的卵巢癌(endometriosis associated ovarian cancers, EAOCs)。

目前认为细胞的异常凋亡和肿瘤的发生关系密切。第10号染色体同源丢失性磷酸酶张力蛋白基因(phosphatase and tension homology deleted on chromosome ten, PTEN)是1997年由Li等［1］研究命名, PTEN基因具有诱导肿瘤细胞凋亡功能。目前研究认为人类多种恶性肿瘤中存在PTEN基因缺失或突变［1, 2］。凋亡调节基因被认为是继原癌基因、抑癌基因之后的第三类癌基因。S……