薛飞 沈照立 沈睿 陈先震

[摘要] 目的 初步探索microRNAR-21（miR-21）与磷酸酶和张力蛋白同源蛋白（PTEN）基因在人脑胶质瘤中的表达及其临床意义。 方法 采用RT-PCR检测215例胶质瘤（胶质瘤组）和50例正常人脑组织（对照组）中miRNA-21的表达水平，采用SP免疫组化法检测PTEN蛋白的表达情况，并探讨二者在胶质瘤组织中表达的相关性。 结果 miR-21在胶质瘤组中的相对表达量（2-ΔΔCT）（4.222±1.755）明显高于对照组织（1.064±0.126），差异有统计学意义（P < 0.05）。PTEN在对照组中的表达率（100.00%）高于胶质瘤组（49.30%），差异有统计学意义（P < 0.05）。胶质瘤组中miR-21的表达量与PTEN在胶质瘤组中的阳性表达率呈负相关（r=-0.448，P < 0.05）。 结论 miR-21在胶质瘤组织中的表达升高，可能通过负性调节PTEN的表达，在胶质瘤的发生发展中发挥作用，二者的表达可以显示胶质瘤的恶性程度，通过检测二者在胶质瘤中的表达有助于评价胶质瘤的恶性程度和生物学行为。

[关键词] microRNAR-21；磷酸酶和张力蛋白同源蛋白；脑胶质瘤；相关性

[中图分类号] R739.41[文献标识码] A[文章编号] 1673-7210（2014）04（c）-0036-04

Expression of micro RNA-21 and phosphatase and tensin homology gene in glioma and their clinical significances

XUE Fei SHEN Zhaoli SHEN Rui CHEN Xianzhen

Department of Neurosurgery, the 10th People's Hospital Afflilated to Tongji University, Shanghai 200072, China

[Abstract] Objective To investigate the expression of microRNAR-21 (miR-21) and phosphatase and tensin homolog deleted on chromosome ten (PTEN) in glioma and their clinical significances. Methods The expression of miR-21 was detected by RT-PCR in 215 glioma (glioma group) and 50 cases of normal brain tissues (control group). S-P immunohistochemical was used to detect the expression of PTEN. Results The expression of miR-21 (2-ΔΔCT) in the glioma group was (4.222±1.755), which was significantly higher than that in the control group (1.064±0.126), the difference was statistically significant (P < 0.05). The positive rate of PTEN protein in glioma group (49.30%) was lower than that in control group (100.00%), the difference was statistically significant (P < 0.05). There was negative correlation between miR-21 and the positive ratio of PTEN (r=-0.448, P < 0.05). Conclusion miR-21 might play an important role in the down-regulation of PTEN and development of glioma, the expression of two show the m alignant degree of glioma, it is helpfal to evaluate the m alignant degree and biologic behavior of glioma by detecting both expression.

[Key words] miR-21; PTEN; Glioma; Correlation

微小RNA（microRNA）是真核生物中一种长度约为20~22个核苷酸的非编码小分子RNA，通过特异性识别并结合靶mRNA的3'非编码区（3'UTR）使靶mRNA降解，发挥其对基因的转录后调控，在细胞的生长、分化、凋亡及代谢等活动中发挥着重要作用[1]。最近几年的研究发现，MicroRNA在肿瘤的发生、发展和转移中同样起到非常重要的作用[2]，并有大量实验证据表明，微小RNAR-21（miR-21）在结肠癌、乳腺癌和膀胱癌等肿瘤细胞中的表达都表现出显著异常[3-5]，在胶质瘤表达的microRNA中，miR21表达水平升高最为明显[6]。通过计算机预测软件并经实验证实的miR21的靶基因包括：张力蛋白同源（PTEN）、PDCD4、RECK、TIMP3、Fasl、TRIP、Tap6、HNRPK及Spry2， 这些基因都发挥着抑制肿瘤生长的作用[7]。……