唐兴国等

摘要[目的]对紫薇幼胚培养进行尝试性研究，探讨其适宜的生长分化条件。[方法]以紫薇（Lagerstroemia indica）幼胚为外植体，诱导其萌发并建立无菌系。在此基础上探讨了不同激素水平和培养方式对紫薇幼胚培养的影响。[结果]紫薇幼胚去种皮后易于萌发，其萌发率可达100%；启动培养基为 MS+ BA0.5+ NAA0.5+ sucrose3.0%+ agar0.7%；丛生芽诱导培养基为MS+ BA0.5 + NAA0.1+ sucrose3.0%+ agar0.7%+ 椰乳10%；生根培养基为MS+ BA0.5 + IBA0.1+ sucrose3.0%+ agar0.7%+ 椰乳10%。[结论]该研究可为紫薇的后续优化育种提供技术参考。

关键词紫薇；幼胚；组织培养

中图分类号S603.6；Q813.1文献标识码A文章编号0517-6611（2014）36-13177-02

Abstract[Objective] This study aimed to explore immature embryo culture of Lagerstroemia indica and investigate the appropriate conditions for growth and differentiation. [Method] Immature embryos of L. indica were employed as the explants for germination induction to establish aseptic lines. Based on that， the effects of different hormone levels and culture contions on immature embryo culture of L. indica were analyzed. [Result] Peeled immature embryos of L. indica were easy to germinate， leading to a germination rate of 100%. The optimal initial medium was MS+ BA0.5+ NAA0.1+ sucrose3.0%+ agar0.7%； the optimal shoot induction medium was MS+ BA0.5 + NAA0.1+ sucrose3.0%+ agar0.7%+ coconut milk 10%； the optimal rooting medium was MS+ BA0.5+ IBA0.1+ sucrose3.0%+ agar0.7%+ coconut milk 10%. [Conclusion] This study provided technical reference for subsequent optimized breeding of L. indica.

Key wordsLagerstroemia indica； Immature embryo； Tissue culture

紫薇（Lagerstroemia indica）为千屈菜科紫薇属木本花卉，原产于中国[1]，是我国传统名花之一，花开在夏季少花季节，是优良的园林观赏花木，亦是良好的树桩盆景植物[2]。紫薇的病害较多，最常见的有白粉病、褐斑病、煤污病等，主要为真菌性病害[3]，病害直接影响紫薇的植株生长态势和观赏效果，因此培育无病或抗病紫薇植株一直是园艺工作者的夙愿[4]。前人研究表明，通过组织培养方式培育紫薇幼株，可在一定程度上解决紫薇感染病害的问题，并可进行批量繁殖[5]。目前已报道文献中，紫薇组织培养取材以幼嫩茎段为主[6-8]，而以幼胚作为外植体进行离体培养方式目前尚未见报道。通过幼胚离体培养，可以进一步提高紫薇的抗病性，同时具有克服杂种胚败育、获得稀有品种、缩短育种周期等优点。该试验对紫薇幼胚培养进行尝试性研究，目的在于探讨其适宜的生长分化条件，为后续的优化育种提供技术参考。

1材料与方法