乔小东+武华

乔小东1 武 华2

1.山西医科大学，山西太原 030001；2.山西医科大学第一临床医学院普外科，山西太原 030001

[摘要] 目的 研究复合膳食纤维对门静脉高压大鼠肠黏膜屏障功能的保护作用。 方法 健康雌性Wister大鼠，通过四氯化碳（CCl4）橄榄油复合法建立门静脉高压大鼠模型，建模成功后随机分为A组（单纯模型组）、B组（能全素组）、C组（能全素+膳食纤维组，可溶与不可溶之比为1∶2）、D组（能全素+膳食纤维组，可溶与不可溶之比为2∶1）。分别于建模后1、3、7 d随机取各组3、3、4只大鼠的回肠组织在光镜下观察其病理形态变化，取腹主动脉血检测血浆D-乳酸的表达，取门静脉血检测血浆内毒素的表达。另设正常对照组，同法于第7天取样进行检测。 结果 建模后各组大鼠的肠黏膜组织结构均有损伤，对照组无损伤；血浆D-乳酸和内毒素表达明显高于对照组，实验组建模后1 d四组组间比较，差异均无统计学意义（血浆D-乳酸：F = 0.579，P = 0.633；内毒素：F = 0.108，P = 0.955）；3、7 d四组组间比较，差异均高度有统计学意义（血浆D-乳酸3 d：F = 39.622，P < 0.001，7 d：F = 203.834，P < 0.001；内毒素3 d：F = 95.863，P < 0.001，7 d：F = 2742.321，P < 0.001），且3、7 d各时相B、C、D组大鼠血浆D-乳酸表达和内毒素表达分别与A组比较，差异均有统计学意义（P < 0.05），3、7 d C、D组分别与B组比较，差异均有统计学意义（P < 0.05），7 d D组与C组比较，差异有统计学意义（P < 0.05）。 结论 复合膳食纤维对门静脉高压大鼠肠黏膜屏障功能有一定的保护作用。

[关键词] 复合膳食纤维；门静脉高压；肠黏膜屏障；D-乳酸；内毒素

[中图分类号] R151 [文献标识码] A [文章编号] 1673-7210（2014）03（c）-0026-04

Detection of D-lactic acid and endotoxin and effect of dietary fiber complex on intestinal mucosal barrier in rats with portal hypertension

QIAO Xiaodong1 WU Hua2

1.Shanxi Medical University， Shanxi Province， Taiyuan 030001， China； 2.Department of General Surgery， the First Clinical Medical College of Shanxi Medical University， Shanxi Province， Taiyuan 030001， China

[Abstract] Objective To research the protective effect of dietary fiber complex （DFC） on intestinal mucosal barrier in rats with portal hypertension. Methods The healthy female Wister rats with portal hypertension which were established by CCl4 olive oil complex method were randomly divided into four groups： group A （simple model group）， group B （the rats were fed with Nutrison）， group C （the rats were fed with Nutrison and DFC， SDF∶IDF=1∶2）， group D （the rats were fed with Nutrison and DFC， SDF∶IDF=2∶1）. At 1， 3， 7 d after modeling， 3， 3， 4 rats in each group were randomly selected ileum tissue to observe the pathological changes in the light microscope. Blood samples were taken for D-lactic acid content and endotoxin content. And the normal control group was sampled at 7 d as the tested group. Results The organizational structure of intestinal mucosa of rats in each experimental group were damaged， the control group were no damage. The levels of D-lactic acid and endotoxin in the blood were significantly higher than those in the control. At 1 d after modeling， the differences were not statistically significant in tested groups （plasma D-lactate： F = 0.579， P = 0.633； endotoxin： F = 0.108， P = 0.955）. At 3 d and 7 d， the differences in tested groups were statistically significant （plasma D-lactate 3 d： F = 39.622， P < 0.001， 7 d： F = 203.834， P < 0.001； endotoxin 3 d： F = 95.863， P < 0.001， 7 d： F = 2742.321， P < 0.001）. Compared with group A， the differences in the levels of D-lactic acid and endotoxin in the group B， C and D in 3， 7 d were significant （P < 0.05）， the levels significantly reduced. Compared with B， the differences were significant in the group C and D in 3， 7 d （P < 0.05）. Compared with group C， the difference were significant in the group D in 7 d （P < 0.05）. Conclusion DFC has a protective effect on gut barrier in rats with portal hypertension.

[Key words] Dietary fiber complex； Portal hypertension； Intestinal mucosal barrier； D-lactic acid； Endotoxin

门静脉高压症（portal hypertension，PHT）是临床一种常见病、多发病，为各种原因所致门静脉血循环障碍而导致的一系列临床综合征，病死率较高，影响患者的生命质量，并对家庭和社会造成严重的负担。……