吴 春，杜成琨

（哈尔滨商业大学 食品工程学院，黑龙江 哈尔滨 150076）

木犀草素酯化物的制备及其抗氧化活性研究*

吴 春，杜成琨

（哈尔滨商业大学 食品工程学院，黑龙江 哈尔滨 150076）

利用月桂酰氯酯化制备了脂溶性木犀草素。利用正交实验研究了反应物配比、反应温度及反应时间对制备木犀草素酯化物的影响。通过红外光谱对酯化物进行结构表征，并考察木犀草素酯化物在大豆油中的溶解性和抗氧化活性。制备木犀草素酯化物的最佳反应条件是：反应物配比n(木犀草素)∶n(月桂酰氯)=1∶2、反应温度50℃、反应时间5h，其在大豆油中的溶解度为2.02×104μg/mL，且在相同浓度下，抗氧化活性优于二丁基羟基甲苯(BHT)。结果表明：木犀草素酯化物在大豆油中的溶解度明显提高，并具有良好的抗氧化性能。

木犀草素;酯化;抗氧化活性

Abstract:In this experiment,lipid soluble luteolin had been synthesized by esterification of lauroyl chloride.The effects of reactants ratio,reaction temperature and time on the preparation of luteolin esters were studied by using the orthogonal experiments.And the structure of the prepared product was characterized by IR spectrum.The solubility and antioxidation properties of the product in soybean oils were examined.The optimal reaction conditions were showed as follows:the molar ratio of luteolin to lauroyl chloride was 1∶2 at 50℃,reaction time was 5h and reaction temperature was 50℃.The solubility of the product in the soybean oils was 2.02×104μg/mL.Under the condition of same concentration,the antioxidation activity of the luteolin laurate was better than BHT in oils.The solubility of the product in soybean oils was improved obviously and the product had good antioxidation properties.

Key words:Luteolin;esterification;antioxidation activity

前 言

木犀草素的化学名为3’,4’,5,7-四羟基黄酮(3’,4’,5,7-Tetralydroxyflanone)，化学式为 C15H10O6[1]。木犀草素是一种广泛存在的天然黄酮，具有很强的抗氧化性及抗炎、抗癌作用[2]。木犀草素具有良好抗脂质氧化能力，其最适添加量为0.02%左右。标准程序的化学分析研究表明，等摩尔浓度条件下的木犀草素与槲皮素及辣椒素相比，木犀草素具有最强的抗氧化活性[3]。

由于木犀草素难溶于油脂中，限制了其作为天然抗氧化剂的应用。我国许多食用色拉油很容易氧化，即使未出厂贮存在加工厂的贮罐中，一般不超过3个月其POV值都已超标[4]，研发脂溶性木犀草素以应用到食用油脂中，将大大拓展木犀草素的应用市场。……