陈 钰(Chen Yu， Dept Med Res，Guangzhou Gen Hosp，Guangzhou Mil Command，Guangzhou，Guangdong 510010)…∥Chin J Clin Basic Orthop Res.－2010，2(3).－226～230

Design of nano-piezoelectric immunosensor array to detect bone sialoprotein

陈 钰(Chen Yu， Dept Med Res，Guangzhou Gen Hosp，Guangzhou Mil Command，Guangzhou，Guangdong 510010)…∥Chin J Clin Basic Orthop Res.－2010，2(3).－226～230

ObjectiveWith the unique properties of Au nanoparticles，a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein(BSP)expression from clinical serum.MethodsMonoclonal antibody of BSP was thiolated by heterobifuncfional cross-linkers Sulfo-LC-SPDP.Next，the hydrosulfuryl-antibodies were fixed on the surface of gold-membrane electrode via Au nanoparticles modified to make a bio-sensitive membrane，which constructed the nano-piezoelectric immunity chips.BSP expression from the serum of healthy adults and patients with osteoporosis were examined alter immunoreaction.ResultsThe detection of reference standard serum at dilution of 1∶9，1∶8，1∶7，1∶6，1∶5，1∶4，1∶3 and 1∶2 showed that constructed BSP nano-immunosensor array had good linear relationship with serum detection results when the serum dilution ranged between 1∶9 and 1∶2;the linear equation was y=872.66 x－58.258(correlation coefficient=0.974).The serum BSP expression levels of patients with osteoporosis were higher than those of healthy adults using BSP piezoelectric immunosensor array(t=20.162，P=0.000).ConclusionThe BSP nano-piezoelectric immunosensor array can be used for quantitative detection of serum BSP with the advantages of simple structure，easy operation，good stability，high sensitivity and specificity.23 refs，3 figs.

(Authors)