徐志锋 李 萍 浦亚红 杨世玉 杜荣琼 张 烨

摘要采用紫外-可见分光光度法检测大理杜鹃花黄色素的提取效果。选用单因素试验和正交试验法对时间、料液比、浓度、温度这4个因素进行考察,以确定最佳工艺。结果表明,采用60%乙醇作为提取剂、提取时间60 min、提取温度60 ℃,料液比1∶80是提取大理杜鹃花色素较适宜的工艺条件。该色素在pH值≤7时较稳定,在60 ℃下热稳定性好,光稳定性较差,应避光保存;除Fe3+外其对常见金属离子有一定的稳定性。该工艺操作简单、合理可行。

关键词 大理杜鹃花;黄色素;提取工艺;稳定性

中图分类号 TS202.3 文献标识码A文章编号 1007-5739(2010)15-0011-02

StudyonExtractionTechnologyandPropertyofYellowPigmentinRhododendrontaliewseFranch

XU Zhi-fengLI PingPU Ya-hongYANG Shi-yuDU Rong-qiongZHANG Ye*

(Pharmacy College of Dali University,Dali Yunnan 671000)

AbstractUv-Vis was developed for the determination of extraction effect. 4 factors that might influence the extraction rate were examined by single factor and orthogonal experiments to select the most optimum extracting technology of Rhododendron taliewse Franch yellow pigment. The 4 factors were times of extraction,the ratio of material and water solvent and extraction temperature. The results showed that the best extraction technology was 60% of ethanol,extraction for 60 min,60 ℃ of the extraction temperature,1∶80 of the ratio of material and water. The pigments stability was in pH≤7,it has the good thermal stability under 60 ℃,its light stability was poor and its stability for most mental ions except Fe3+. This extraction technology was rational,simple,stable and feasible.

Key wordsRhododendron taliewse Franch;yellow pigment;extraction technology;stability technology;stability

合成色素由于安全问题,在使用上已逐渐受到控制。因此,寻找和开发更多的天然色素已成为食品或化妆品色素发展的趋势[1-3]。大理杜鹃(Rhododendron taliense Franch),杜鹃花科杜鹃花属常绿灌木,分布于四川西部、云南西部及西北部。研究证明,杜鹃花属植物在祛痰、止咳平喘、心血管系统、神经系统、抗炎镇痛、免疫、杀虫等方面具有药理活性[4-5]。本试验系统地研究了大理杜鹃花色素的提取工艺并对其性质进行初步研究,以期为该色素的开发和利用提供参考。

1材料与方法

1.1材料与试剂

试验材料采自云南省大理苍山,经大理学院药学院马晓匡教授鉴定为大理杜鹃(Rhododendron taliense Franch)。将大理杜鹃花阴干、粉碎后备用。试验中所用试剂石油醚、苯、乙酸乙酯、丙酮、无水乙醇、三氯化铁、氯化钠、无水氯化钙、无水硫酸铝、无水硫酸锌、EDTA等试剂均为分析纯。

1.2仪器

SSY型电热恒温水浴……