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【摘要】 目的 了解合山市某乡成年人群肝胆患病情况，以利于肝胆疾病的积极防治。方法 用酶联免疫试验检测2 761名成人自愿参检者血清HBsAg、AFP；用赖氏法检测ALT；用B超检查肝、胆。 结果 HBsAg 阳性者有283例(10．25%)、脂肪肝158例(5．72%)、ALT升高32例(1．16%)、AFP升高22例(0．80%)、肝血管瘤14例(0．51%)。男性HBsAg 阳性及脂肪肝患病率均显著高于女性(P<0．001)，而肝血管瘤患病率却显著低于女性(P<0．05)。结论 该地区人群中肝胆疾病患者比例是相当大的，人群的健康普查是必要的。

【关键词】肝胆疾病；患病率；调查

The state of liver and gallbladder illness in 2761 rural residents

QIN Guo-de.Lingnan Hospital,Heshan,Guangxi 546506，China

【Abstract】 Objective To explore the state of liver and gallbladder illness in countrymen of Heshan Town.Methods The sera from 2761 people were detected for Hepatitis B virus surface antigen(HBsAg)and Alpha-fetoprotein(AFP)were detected with enzyme-linked immunosorbent assay(ELISA).ALT was detected by Lais method.Liver and Gallbladder were examined by ultrasonic diagnostics.Results 283 cases of 2761 were positive for HBsAg,158 cases were suffered from Fatty Liver,ALT and AFP were higher than normal levels in 32 and 22 cases respectively.14 cases of liver hoemangioma were found.Positive rate of HBsAg in men was significantly higher than that in female(P<0．001)and the prevalence rate of liver hoemangioma was significantly lower in men(P<0．05).Conclusion The prevalent rates of liver and gallbladder illness were high in this region.It is necessary to healthiness examination of rural residents.

【Key words】Liver and gallbladder illness; Prevalent rate; Investigate

人群的健康检查是了解当地人们健康情况最有效的方法。是提高群众的健康水平及建立居民健康档案，健全人人享受卫生保健的基本措施。获李嘉诚医疗扶贫救助基金会等基金资助，作者对合山市某乡2761名成人自愿参检者进行了体检，并将调查结果进行分析。

1 对象与方法

1．1 对象 某乡男25~55岁、女25~50岁自愿参检者。

1．2 材料 HBsAg及AFP酶联免疫试剂盒均由上海科华生物技术有限公司生产， ALT试剂盒由上海荣盛生物技术有限公司提供。

1．3 方法

1．3．1 HBsAg检测按说明书程序进行操作。标本孔加入待测标本血清0．05 ml，每孔再加入酶联试剂1滴混匀，置37℃孵育30 min，取出洗涤完毕，每孔加显色剂A液、B液各1滴混匀，置37℃孵育l5 min，判断结果。

1．3．2 血清谷丙转氨酶作用于两氨酸及a-酮戊二酸，在一定条件下产生一定量的丙酮酸，在酶反应到达规定的时间，加入2,4-二硝基苯肼，在酸性条件下终止酶反应，2,4-二硝基苯肼分别与产物丙酮酸及剩余基质α-酮戊二酸形成各自的二硝基苯腙，碱性条件下两种苯腙均显红棕色，比色后查阅赖氏法标准曲线得出A酶的活力。

1．3．3 血清AFP测定 ELISA法，操作严格按照试剂盒说明书进行。……