王松松　黄慧珍　苏陈颖　林凡　黄明辉　方敏

[关键词] 网络药理学;山奈酚;2型糖尿病;治疗机制

[中图分类号] R285          [文献标识码] A          [文章编号] 1673-9701（2021）22-0017-03

Research on the mechanism of kaempferol involving in Xuefu Zhuyu Decoction in the treatment of type 2 diabetes mellitus based on network pharmacology

WANG Songsong   HUANG Huizhen   SU Chenying   LIN Fan   HUANG Minghui   FANG Min

College of Integrated Traditional Chinese and Western Medicine， Fujian University of Traditional Chinese Medicine，Fuzhou   350122， China

[Abstract] Objective To investigate the therapeutic mechanism of kaempferol， an important active component of Xuefu Zhuyu Decoction， in the treatment of type 2 diabetes mellitus （T2DM） by means of network pharmacology. Methods The action target of kaempferol was obtained from the public database of traditional Chinese medicine systems pharmacology （TCMSP）， and the related genes of T2DM were searched by GeneCards database， and the intersection of them was taken as the anti-T2DM target of kaempferol. Then， a protein-protein interaction （PPI） network of "nti-T2DM target of kaempferol" was constructed by using STRING platform， and the network topology was analyzed by using Cytoscape 3.7.1 software. Then GO and KEGG enrichment analysis of anti-T2DM target protein of kaempferol was carried out by Omishare platform. Results A total of 54 possible anti-T2DM action target of kaempferol were obtained， among which 6 core anti-T2DM target of kaempferol were AKT1，JUN， PTGS2， TNF， CASP3 and MAPK8. Through GO enrichment analysis， 2831 GO items were obtained， including 2355 items in biological process， 181 items in cell composition， and 295 items of molecular function. Through KEGG enrichment analysis， 140 pathways were obtained（P<0.05）， including AGE-RAGE，TNF，IL-17 and C-type lectin receptor and other signaling pathways. Conclusion Kaempferol may regulate signaling pathways such as AGE-RAGE， TNF and IL-17 through AKT1， TNF and other core targets， and involve in the role of Xuefu Zhuyu Decoction in promoting blood circulation and removing blood stasis and treating T2DM.

[Key words] Network pharmacology; Kaempferol; Type 2 diabetes mellitus; Therapeutic mechanism

T2DM患病率逐年增长且给全球带来巨大的经济负担。据报道，2010—2030年全球成年人患糖尿病的数量将从2.85亿增加到4.39亿，增长54%[1]。T2DM患者常伴有并发症，如心血管疾病、糖尿病性神经病、肾病和视网膜病变等，显著降低患者的生活质量，探寻T2DM的治疗新药具有重要意义[2]。近年来，中医药治疗糖尿病的优势日益受到关注，祛瘀活血为其重要治则之一。作为活血化瘀经典方——血府逐瘀汤治疗T2DM疗效显著，但机制尚不明确[3]。山奈酚是血府逐瘀汤中的一种重要活性成分，具有抗氧化、抗炎、抗癌和保护神经等作用[4-5]。它能促进骨骼肌葡萄糖代谢，抑制肝糖异生[6]，改善细胞胰岛素敏感性[7]，激活胰腺β细胞的胰岛素分泌，具有治疗糖尿病的作用[8]。因此，山奈酚可能是血府逐瘀汤改善患者血流、益气活血、化瘀养阴的重要活性成分，但具体的分子机制有待进一步研究。本研究通过网络药理学方法分析山奈酚作用靶標，探讨山奈酚参与血府逐瘀汤治疗T2DM的作用机制，为临床应用和后续研究提供参考，现报道如下。