Adipose-brain crosstalk: do adipokines have a role in neuroprotection?

Accumulating evidence from epidemiological and experimental studies indicate that obesity, and its related metabolic consequences of insulin resistance and type 2 diabetes, are associated with accelerated cognitive decline (Yates et al., 2012). The etiology of neurodegeneration in obesity is undoubtedly complex, with vascular, metabolic, infl ammatory, and structural changes all likely to play a role (Yates et al., 2012). The discovery of leptin in 1994 and the subsequent advancement in our understanding that adipose tissue is an endocrine organ that can communicate with the brain to regulate appetite (Zhang et al., 1994) brings about the intriguing possibility that adipose-brain crosstalk can regulate aspects of neuronal physiology and pathology (Aguilar-Valles et al., 2015). Indeed neurons have been shown to express receptors for various adipokines, indicating that factors released from adipose tissue have the potential to communicate directly with the brain. Research in this area is relatively new, and while epidemiological data points towards the negative consequences of adipose-brain crosstalk (Whitmer et al., 2005), some intriguing new studies highlight that the secretory profi le of adipose tissue might be involved in reduction in neurodegeneration via maintenance of neuronal viability (Tezapsidis et al., 2009; Wan et al., 2015).

Obesity is accompanied by infl ammation in adipose tissue. Much of this infl ammation is driven by infi ltration of macrophages and other immune cells, although adipocytes themselves can also secrete pro-inflammatory cytokines (termed “adipokines”). By virtue of its elevated mass, pro-infl ammatory secretions from macrophage-infi ltrated adipose tissue are believed to contribute to systemic lowgrade inflammation, insulin resistance, and vascular dysfunction in obesity (Ouchi et al., 2011). Increased expression of pro-infl ammatory cytokines and activation of infl ammatory responses are prominent in brain of Alzheimer’s disease (AD) and aging-related dementia patients. Rodent models of obesity demonstrate elevated markers of brain infl ammation and oxidative stress (Pistell et al., 2010) and a recent study has supported that humans with obesity have elevated markers of brain infl ammation in hypothalamus assessed in vivo (Thaler et al., 2012). On the other hand, both in vitro and in vivo studies have reported that adipokines like leptin can mitigate systemic and central nervous system molecular pathologies associated with AD (Greco et al., 2009; Chakrabarti et al. 2015). Taken together, these fi ndings provide speculative support that secretions from adipose tissue may impact brain infl ammation and subsequently alter the risk of neurodegeneration in obesity. This also warrants immediate attention to profi le the secretome based on the source of adipose tissue (lean vs. obese mouse models or human subjects), which may determine positive or negative adipose-brain cross talk.

Direct experimental evidence for adipose-brain crosstalk in vivo presents many technical challenges, particularly in humans. Recently, Wan et al. (2015) performed a series of experiments using an in vitro model of adipose-brain crosstalk in attempts to shed light on possible communication between human adipose tissue and neurons. Adipose tissue organ cultures, which retain in vivo secretory profi le and intercellular communication between adipocytes and immune cells, were prepared from human donors. Adipose tissue organ culture (ATOC) conditioned media, containing the full ensemble of secretory products, was applied to human SH-SY5Y neuronal cells that were left untreated or treated with hydrogen peroxide (H2O2) to induce oxidative stress-induced cell death. ATOC conditioned media obtained from lean subjects had no eff ect on SH-SY5Y cell viability in the untreated condition but when neuronal cells were exposed to H2O2they were almost completely protected from oxidative stress-induced cell death. However, ATOC conditioned media from obese donors lacked this protective eff ect. Since oxidative stress has been shown to drive neurodegenerative processes in AD and related dementias (Perry et al., 2002), the protective eff ect of lean ATOC conditioned media on SH-SY5Y neuronal cells suggests that some factor(s) secreted from lean adipose tissue may possess neuroprotective properties. Unfortunately the authors were unable to isolate what factor(s) were involved but they did show that when ATOC conditioned media was heated to denature proteins (10 minutes at 95°C) the neuroprotective eff ects were lost, implicating the peptide nature of the putative neuronal pro-survival adipokine(s).

These preliminary fi ndings suggest that adipokines can regulate adipose-brain crosstalk and can play a role in neuroprotection or neurodegeneration depending on the adiposity status of the individual. Particularly, the results indicate that lean adipose tissue may secrete certain adipokines that are protective towards neurons whereas in obesity, adipose tissue may lack this protective potential. It appears that proteins or peptides are involved but it remains to be determined which adipokine(s) may possesses these neuroprotective properties.

It must be noted that these fi ndings were obtained in an ex vivo-in vitro model system of adipose-brain crosstalk, so the results may not be entirely applicable to the much more complex system in vivo, where adipose secretions would interact with multiple diff erent cell types and have to cross the blood-brain barrier prior to eliciting any eff ect on neurons. It seems clear that adipokines can cross the blood brain barrier, as shown by the classic fi ndings involving leptin and appetite regulation (Zhang et al., 1994). However, the concentrations of adipokines reached in brain areas relevant to AD and dementia (e.g., hippocampus) are currently unclear. Future research confi rming the potential benefi ts of lean adipose tissue secretions on neuronal cell viability is warranted. In this regard, the adipose tissue transplantation model developed by Kahn and colleagues (Tran et al., 2008) would seem to be an ideal model to test this in vivo. Much like leptin treatment, lean adipose tissue transplantation into obese mice has been shown to reverse metabolic defects and improve glucose tolerance (Tran et al., 2008); could transplantation of lean

adipose tissue prevent brain infl ammation and accelerated cognitive decline in obese animals? In addition, given the key role of glial cells in propagating brain infl ammation in neurodegenerative diseases, the impact of adipokines on glial cell function warrants further investigation. An alternative line of research stemming from these fi ndings could also include identifying the putative neuroprotective factor(s) secreted from lean adipose tissue for drug discovery purposes.

In summary, the recent fi ndings of Wan et al. (2015) provide intriguing preliminary evidence that lean adipose tissue may secrete factors that possess neuroprotective properties. Confi rmation of these fi ndings in vivo, and further exploration into their identity, may enhance our understanding of how adipokines mediate adipose-brain cross-talk and provide new therapeutic targets to help protect neurons from damage.

Jonathan P. Little*, Adeel Safdar

School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, BC, Canada, V1V 1V7 (Little JP) Departments of Pediatrics and Medicine, McMaster University, Hamilton, ON, Canada, L8S 4K1 (Safdar A)

*Correspondence to: Jonathan P. Little, Ph.D.,

jonathan.little@ubc.ca.

Accepted: 2015-06-15

orcid: 0000-0002-9796-2008 (Jonathan P. Little)

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10.4103/1673-5374.165222 http://www.nrronline.org/ Little JP, Safdar A (2015) Adipose-brain crosstalk: do adipokines have a role in neuroprotection? Neural Regen Res 10(9):1381-1382.